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Image Search Results


The antiproliferative activity of the epothilone and glutathione-Epothilone conjugates at 1:2 and 1: 4 M:M, towards the HepG-2 cells and control THLE-2 cells, normalized to Staurosporine as reference drug

Journal: BMC Microbiology

Article Title: Aspergillus flavus glutathione transferase as a potential approach for synthesis of epothilone-glutathione conjugates with enhanced anticancer activity for the drug-resistant cells: characterization and molecular docking analysis

doi: 10.1186/s12866-026-05037-0

Figure Lengend Snippet: The antiproliferative activity of the epothilone and glutathione-Epothilone conjugates at 1:2 and 1: 4 M:M, towards the HepG-2 cells and control THLE-2 cells, normalized to Staurosporine as reference drug

Article Snippet: The activity of constructed Glutathione-Epothilone B conjugate against the liver carcinoma (HepG-2) (ATCC HB-8065), and normal epithelial cells (THLE-2) (ATCC CRL-2706) was determined by the MTT reagent [ ].

Techniques: Activity Assay, Control

Cell viability assays were conducted using the WST-1 method to evaluate the effects of valproic acid (VPA) and bempedoic acid (BA), alone or in combination, on the viability of THLE-2, Hep3B, and Huh7 cells. (A) The outlines of the WST-1 assay protocol were followed in the study. The effects of (B) valproic acid (0,1, and 2mM), (C) bempedoic acid (0,10, and 25μM), and (D) their combinations on THLE-2 cells, respectively. Similarly, (E–G) show the effects on Hep3B cells and (H–J) present corresponding data for Huh7 cells. Cell viability was measured at 72 and 96 hours post-treatment, as shown at the top of each plot. Red-highlighted values indicate fold change inhibitory effects compared to the control, where values less than one indicate inhibition and values greater than one represent stimulation. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, ns-non-significant. Analysis was performed using one-way ANOVA. Data are shown as mean ± SD.

Journal: bioRxiv

Article Title: Simultaneous Inhibition of ACLY and OGDH Has a Synergistic Effect on Hepatocellular Carcinoma Cell Lines

doi: 10.64898/2026.04.19.716936

Figure Lengend Snippet: Cell viability assays were conducted using the WST-1 method to evaluate the effects of valproic acid (VPA) and bempedoic acid (BA), alone or in combination, on the viability of THLE-2, Hep3B, and Huh7 cells. (A) The outlines of the WST-1 assay protocol were followed in the study. The effects of (B) valproic acid (0,1, and 2mM), (C) bempedoic acid (0,10, and 25μM), and (D) their combinations on THLE-2 cells, respectively. Similarly, (E–G) show the effects on Hep3B cells and (H–J) present corresponding data for Huh7 cells. Cell viability was measured at 72 and 96 hours post-treatment, as shown at the top of each plot. Red-highlighted values indicate fold change inhibitory effects compared to the control, where values less than one indicate inhibition and values greater than one represent stimulation. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, ns-non-significant. Analysis was performed using one-way ANOVA. Data are shown as mean ± SD.

Article Snippet: The immortalized human liver epithelial cell line THLE-2 and human HCC cell lines Hep3B were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: WST-1 Assay, Control, Inhibition